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KMID : 0364820090450040404
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Korean Journal of Microbiology
2009 Volume.45 No. 4 p.404 ~ p.410
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The Physicochemical Stabilities and Biological Activities of Pigment Extracts from Pseudoalteromonas sp. Ju11-1 and Pseudoalteromonas sp. Ju14
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Park Jin-Sook
Cho Hyun-Hee
Kang Myung-Hee
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Abstract
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We investigated the physicochemical stabilities and biological activities of ethanol- extracted pigment from marine bacteria Pseudoalteromonas sp. Ju11-1 and Pseudoalteromonas sp. Ju14. The bacterial pigment of strain Ju11-1 was very stable at pH 5.0 below 25oC. The stability of the pigment showed higher stability in the presence of metal ions such as Cu2+ and Mg2+. The pigment has activity of free-radical scavenging (IC50 95.2 μg/ml) and the protective antioxidant effect (ED50 82.3 μg/ml) against DNA damage in human lymphocytes. The bacterial pigment of strain Ju14 was very stable at pH range between 4.0 and 8.0 below 40oC. In the presence of light, the pigment was also very stable, showing more than 90 percent of remaining absorbance during 14 days at 25oC. The stability of the pigment, when metal ions were present, showed higher stability in all examined metal ions except for Fe2+, Al3+, and Cu2+, especially in the presence of Na+. The pigment has activity of freeradical scavenging (IC50 208.6 μg/ml) and the protective antioxidant effect (ED50 96.4 μg/m) against DNA damage in human lymphocytes. The result indicates that the bacterial pigments from marine bacteria, Pseudoalteromonas sp. Ju11-1 and Pseudoalteromonas sp. Ju14 showed higher physicochemical stability and significant effects for reduction in oxidative DNA damage. Therefore, the results suggest that these bacterial pigments could be used as a natural colorant having the advantages of antioxidant.
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KEYWORD
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bacterial pigment, comet assay, DPPH, marine bacteria, physicochemical stability, Pseudoalteromonas sp.
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